Comparative Study of Commercial and Synthesized Silver Nanoparticle-Loaded Filters for Antibacterial Performance of Water

Authors

  • Sabah A. Khadhira Ministry of Education, General Directorate of Education Diyala, Diyala, 32001, Iraq

DOI:

https://doi.org/10.26554/ijmr.20264398

Keywords:

Purify Water, Filter Paper, Silver-Loaded Filters, Bacterial Removal Efficiency, Escherichia coli ATCC 25922

Abstract

This study aims to fabricate and optimize silver nanoparticle (AgNPs)-loaded filters for enhanced bacterial removal and water purification. Two types of silver were utilized: commercially available silver (Ag1) and laboratory-synthesized silver (Ag2), prepared via a redox displacement method. The synthesized nanoparticles were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), atomic force microscopy (AFM), and energy-dispersive X-ray spectroscopy (EDS), confirming their nanoscale size, crystalline structure, and elemental composition. Various types of filter papers, including membrane and cellulose-based filters, were prepared with and without silver nanoparticle loading. Structural modifications such as layer separation and adhesive incorporation were also investigated to evaluate their impact on filtration performance. The antibacterial activity of the prepared filters was assessed against Escherichia coli ATCC 25922 using the plate count method.
The results demonstrated that AgNPs-loaded filters exhibited significantly enhanced antibacterial efficiency, achieving complete removal (100%) of E. coli in selected configurations, while unloaded filters showed lower removal efficiencies ranging from 53% to 68%. Water quality analysis revealed that total dissolved solids (TDS), electrical conductivity (EC), and pH remained within acceptable drinking water standards after filtration. Furthermore, atomic absorption spectroscopy (AAS) analysis confirmed that the concentration of silver released into the filtered water was within safe limits, indicating no potential risk to human health. Home-based application tests further demonstrated stable performance and consistent water quality under continuous operation. Overall, the developed AgNPs-loaded filters represent an effective, low-cost, and practical solution for water disinfection, particularly in regions lacking advanced water treatment infrastructure.

References

Adjovu, G. E., H. Stephen, D. James, and S. Ahmad (2023). Measurement of Total Dissolved Solids and Total Suspended Solids in Water Systems: A Review of the Issues, Conventional, and Remote Sensing Techniques. Remote Sensing, 15(14); 3534

Ahsan, A., H. Kamran, A. Malik, M. M. Saeed, H. M. Awan, and H. Qayyum (2022). Bactericidal Effect of Laser Synthesized Silver Nanoparticle-Impregnated Cellulose Paper Against E. coli. Optics & Laser Technology, 154; 108305

American Public Health Association (1926). Standard Methods for the Examination ofWater andWastewater, volume 6. American Public Health Association

Baird, R., E. Rice, and A. Eaton (2017). Standard Methods for the Examination of Water and Wastewaters. American Public Health Association, 23 edition

Binnig, G., C. F. Quate, and C. Gerber (1986). Atomic Force Microscope. Physical Review Letters, 56(9); 930–933

Cappuccino, J. G. and N. Sherman (2011). Microbiology: A Laboratory Manual. Pearson Benjamin Cummings, San Francisco

Cullity, B. and S. Stock (2001). Elements of X-Ray Diffraction. Prentice Hall, 388; 1

Dakal, T. C., A. Kumar, R. S. Majumdar, and V. Yadav (2016). Mechanistic Basis of Antimicrobial Actions of Silver Nanoparticles. Frontiers in Microbiology, 7; 1831

Dankovich, T. A. and D. G. Gray (2011). Bactericidal Paper Impregnated with Silver Nanoparticles for Point-of-UseWater Treatment. Environmental Science & Technology, 45(5); 1992–1998

Deshmukh, A., M. Siddiqui, U. K. Pathan, and U. Dhuldhaj (2020). Microbial Study of Organisms Isolated from Nutritional Fruit Juices Surrounded by Local Fruit Market in Nanded, Maharashtra, India. Biodiversity Journal of Biological Diversity, 21(9); 4240–4246

Egerton, R. F. (2005). Physical Principles of Electron Microscopy, volume 56. Springer

Goldstein, J. I., D. E. Newbury, J. R. Michael, N. W. Ritchie, J. H. J. Scott, and D. C. Joy (2017). Scanning Electron Microscopy and X-Ray Microanalysis. springer

Hussain, M. H., J. J. Jader, and A. S. Hasson (2020). Effect of Bio-Fertilization and Foliar Spraying in the Mustard Seed Content Brassica alba L. from Some Fatty Acids. Executive Edit, 11(4); 25–32

Kamei, A. and B. S. Soori (2026). Water Treatment and E. coli in Drinking Water: Household Responses to (Invisible) Water Quality Risks. PLOS ONE, 21(1); e0331258

Klug, H. P. and L. E. Alexander (1974). X-Ray Diffraction Procedures: For Polycrystalline and Amorphous Materials

Kumar, N., R. K. Salar, R. Kumar, M. Prasad, B. Brar, and V. Nain (2017). Green Synthesis of Silver Nanoparticles and Its Applications—A Review. Nano Trends: A Journal of Nanotechnology and Its Applications, 19(3); 1–22

Lech, M., J. Franczyk, M. Radziemska, A. Sieczka, K. Garbulewski, E. Koda, and Z. Lechowicz (2016). Monitoring of Total Dissolved Solids on Agricultural Lands Using Electrical Conductivity Measurements. Applied Ecology and Environmental Research, 14(4); 285–295

Madani, S. S. (1999). The Application of Membrane Technology for Water Disinfection. Water Research, 33(2); 301–308

Madani, S. S. and A. Rahimpour (2005). Effect of Type of Solvent and Non-Solvents on Morphology and Performance of Polysulfone and Polyether Sulfone Ultrafiltration Membranes for Milk Concentration. Polymers for Advanced Technologies, 16(10); 717–724

Ning, Z., C. Chen, T. Xie, Q. Wang, J. Bai, D. Shao, and B. Cui (2020). Windows of Opportunity for Smooth Cordgrass Landward Invasion to Tidal Channel Margins: The Importance of Hydrodynamic Disturbance to Seedling Establishment. Journal of Environmental Management, 266; 110559

Nowicki, S., Z. R. deLaurent, E. P. de Villiers, G. Githinji, and K. J. Charles (2021). The Utility of Escherichia coli as a Contamination Indicator for Rural Drinking Water: Evidence from Whole Genome Sequencing. PLOS ONE, 16(1); e0245910

Rai, M., A. Yadav, and A. Gade (2009). Silver Nanoparticles as a New Generation of Antimicrobials. Biotechnology Advances, 27(1); 76–83

Shano, A. M., S. A. Khadhir, A. A. Mohammed, S. K. Adnan, and O. A. Ahmed (2023). Structural Properties of Zirconia/Alumina Composites Prepared by Various Techniques. In AIP Conference Proceedings, volume 2834. AIP Publishing, page 090023

Sharan, R. F., M. E. Atallah, Y. T. Sarhan, and M. Y. Abed (2020). Incidence and Risk Factors of Neonatal Mortality at Alarmed Teaching Hospital for Maternity and Childhood: A Cross-Sectional Study. Medico-Legal Update, 20(4); 861–867

Sharma, V. K., R. A. Yngard, and Y. Lin (2009). Silver Nanoparticles: Green Synthesis and Their Antimicrobial Activities. Advances in Colloid and Interface Science, 145(1-2); 83–96

Skoog, D. A., F. J. Holler, and S. R. Crouch (1998). Principles of Instrumental Analysis. Saunders College Publishing, Philadelphia

Tasic, L., D. Stanisic, C. H. N. Barros, L. K. Covesi, and E. R. Bandala (2022). Inactivation of Escherichia coli Using Biogenic Silver Nanoparticles and Ultraviolet (UV) Radiation in Water Disinfection Processes. Catalysts, 12; 430

Welz, B. and M. Sperling (2008). Atomic Absorption Spectrometry. John Wiley & Sons

World Health Organization (2011). Guidelines for Drinking-Water Quality. World Health Organization

Zafar, M. S., S. Ejaz, F. Ahmad, A. H. Ibrahim, S. S. Al-Rawi, F. Manzoor, S. Aziz, S. Iqbal, and M. A. Iqbal (2026). Silver Nanoparticles in Water Disinfection: A Comprehensive Overview on Their Mechanisms, Benefits, and Limitations. Archives of Microbiology, 208(4); 166

Downloads

Published

2026-04-20